The signal was absent in lysates of cells in which OPTN had been knocked out using CRISPR-Cas9, confirming the specificity of this antibody. OPTN was distinctively localized to the retinal ...
Certain antibodies will be best tested using CRISPR-Cas9 to knock out the gene that encodes the target protein, and checking that the antibody no longer binds to anything. Other antibodies might ...
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