Real-time PCR is the favored method for measuring gene ... primer annealing and primer extension. With each cycle the amount of template DNA is doubled until one of the reagents becomes limiting ...
Real-time PCR can also be used to calculate the amount of specific kinds of genetic material other than DNA, such as RNA. This extension of real-time PCR technology, called reverse transcription ...
In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.
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