Prior to the development of PCR in the 1980s, the primary method for producing many copies of a gene was a relatively time-consuming process known as DNA cloning. This technique involved insertion ...

In comparison to conventional PCR, it can be used to detect a much wider range, over 10 7 fold, of starting template concentration. It is also less time-consuming, as it does not require analysis ...

But what is real time RT–PCR? How does it work? How is it different from PCR? And what does this have to do with nuclear technology? Here’s a handy overview of the technique, how it works and a few ...