With in vivo cloning, it is possible to introduce a large pool of DNA sequences into the bacteria and to allow new architectures to emerge and new functions to develop. But creating such ...

Select and verify by sequencing at least six Cm R /Km R bacterial clones for each individual target oligonucleotide when cloning individual shRNAs, or ensure at least threefold coverage when ...

Antibiotic resistance genes were typically isolated by cloning from cultured bacteria or by PCR amplification. Those methods missed potential antibiotic resistance reservoirs because of ...