Protocol for dissection and culture of murine dorsal root …
In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of …
A video protocol for rapid dissection of mouse dorsal root …
Jun 24, 2020 · We have previously published a step-by-step dissection method for the rapid isolation of post-natal mouse DRG. Here, the objective is to extend the protocol by providing …
Protocol for dissection and culture of murine dorsal root …
Mar 19, 2021 · Highlights. •. Localization, dissection, and removal of murine dorsal root ganglia (DRG) •. DRGs are processed into single-cell suspensions for sensory neuron culture. •. …
A simple, step-by-step dissection protocol for the rapid isolation …
Feb 11, 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal …
Dorsal Root Ganglia Isolation and Primary Culture to Study ...
Oct 6, 2018 · With DRG primary cultures, scientists may culture dissociated DRG neurons to monitor biochemical changes in single or multiple cells, overcoming many of the limitations …
Protocol for the isolation and culture of mouse dorsal root …
Dec 15, 2023 · Here, we present a protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications. We describe steps for extracting dorsal root …
A combined protocol for isolation, culture, and patch-clamp …
The dorsal root ganglion (DRG) neurons are crucial in transmitting sensory information from the peripheral nervous system to the central nervous system, including touch, pain, temperature, …
Isolation and Culture of Dorsal Root Ganglia (DRG) from Rodents …
May 26, 2021 · Here, we describe an isolation method using a Percoll gradient and an antimitotic reagent to significantly reduce the nonneuronal cell contamination while maintaining the …
A simple, step-by-step dissection protocol for the rapid isolation …
Feb 11, 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal …
An integrated cell isolation and purification method for rat …
Highly purified, stable DRG neurons could be easily harvested and grown for extended periods by using this integrated cell isolation and purification method, which may help to elucidate the …