Fast 3D dark-field reflection-mode photoacoustic microscopy
We present an in vivo dark-field reflection-mode photoacoustic microscopy system that performs cross-sectional (B-scan) imaging at 50 Hz with realtime beamforming and 3D imaging consisting of 166 B-scan frames at 1 Hz with post-beamforming.
Photoacoustic microscopy - Wikipedia
By ignoring ballistic light, dark-field confocal photoacoustic microscopy reduces surface signal. This method uses a dark-field pulsed laser and high-NA ultrasonic detection, with the fiber output end coaxially aligned with the focused ultrasound transducer.
Photoacoustic Microscopy - PMC - National Center for …
Sep 1, 2013 · The dark-field configuration can greatly reduce the surface signals (e.g., from melanin in the epidermis of human skin), and facilitate confocally aligning the optical and acoustic beams. In addition to the dark-field AR-PAM, fiber-based …
Fast 3-D dark-field reflection-mode photoacoustic microscopy in …
We present an in vivo dark-field reflection-mode photoacoustic microscopy system that performs cross-sectional (B-scan) imaging at 50 Hz with real-time beamforming and 3-D imaging consisting of 166 B-scan frames at 1 Hz with postbeamforming.
In vivo dark-field reflection-mode photoacoustic microscopy
Mar 15, 2005 · Reflection-mode photoacoustic microscopy with dark-field laser pulse illumination and high-numerical-aperture ultrasonic detection is designed and implemented in noninvasively imaged blood vessels in the skin in vivo.
Review on practical photoacoustic microscopy - ScienceDirect
Sep 1, 2019 · In this review, we provide practical methods for implementing a PAM system to improve the resolution, signal-to-noise ratio (SNR), and imaging speed. In addition, we review the preclinical and the clinical applications of PAM and discuss the current challenges and the scope for future developments. 1. Introduction.
An Adjustable Dark-Field Acoustic-Resolution Photoacoustic …
Dark-field AR-PAM systems increase resolution by using lens systems to create dark-field illumination that avoids illuminating more superficial areas of the tissue that can produce interfering signals.
Photoacoustic tomography: fundamentals, advances and prospects
Oct 25, 2011 · (A) Schematic diagram of a dark-field acoustic-resolution photoacoustic microscope (AR-PAM). A transverse resolution of 15 µm and axial resolution of 45 µm are achieved, together with the maximum penetration depth of 3 mm in live animals.
Rapid and label-free histological imaging of unprocessed surgical ...
Jan 20, 2023 · Routine examination for intraoperative histopathologic assessment is lengthy and laborious. Here, we present the dark-field reflectance ultraviolet microscopy (DRUM) that enables label-free imaging of unprocessed and thick tissues with subcellular resolution and a high signal-to-background ratio.
Dark-field confocal photoacoustic microscopy - Northwestern …
Photoacoustic microscopy (PAM) [1-3] is a recently developed modality of photoacoustic (PA) imaging. Similar to other PA imaging technologies, PAM detects laser-induced ultrasonic waves (referred to as PA waves) to image the internal distribution of optical energy deposition (specific optical absorption) in biological tissue.