An open-access volume electron microscopy atlas of whole cells …
Oct 6, 2021 · Open-access 3D images of whole cells and tissues with combined finer resolution and larger sample size are enabled by advances in focused ion beam-scanning electron microscopy.
Plasma cell - Wikipedia
Plasma cells are large lymphocytes with abundant cytoplasm and a characteristic appearance on light microscopy. They have basophilic cytoplasm and an eccentric nucleus with heterochromatin in a characteristic cartwheel or clock face arrangement.
Electron Microscopic Analysis of the Plasma Membrane and Cell …
Nov 22, 2017 · In this chapter, we will focus on the current strategies of the plasma membrane preservation, applications of transmission electron transmission (TEM) in studying plasma membrane structure and provide protocols of membrane sample preparation for TEM.
Histology, Plasma Cells - StatPearls - NCBI Bookshelf
Dec 30, 2022 · Plasma cells are differentiated B-lymphocyte white blood cells capable of secreting immunoglobulin or antibodies. They play a significant role in the adaptive immune response, being the main cells responsible for humoral immunity.
Plasma Cell (TEM) | Connective Tissue - Histology Guide
Structure of plasma cells in connective tissue (transmission electron microscopy).
The Cell - Histology Guide
Transmission electron microscopy (TEM) produces two-dimensional images of a specimen by imaging a thin section with a beam of electrons. Ultrathin tissue sections are stained with heavy metals (such as osmium tetroxide, uranium or lead salts) to enhance contrast.
An open-access volume electron microscopy atlas of whole cells …
Electron microscopy (EM) uniquely visualizes cellular structures with nanometer resolution. However, traditional methods, such as thin-section EM or EM tomography, have limitations inasmuch as they only visualize a single slice or a relatively small volume of the cell, respectively.
Electron microscopy methods for studying plasma membranes
Electron microscopy allows direct visualization of the underlying organization of cell surface components on a nano-scale. Immuno-gold labelling of isolated plasma membranes generates point patterns that enable mapping of protein and lipid distributions. 2D spatial statistics reveals the extent to w …
Whole-cell organelle segmentation in volume electron microscopy
Oct 6, 2021 · Focused ion beam scanning electron microscopy (FIB-SEM) combined with deep-learning-based segmentation is used to produce three-dimensional reconstructions of complete cells and tissues, in...
Electron Microscopy Methods for Studying Plasma Membranes
Jan 1, 2014 · Electron microscopy allows direct visualization of the underlying organization of cell surface components on a nano-scale. Immuno-gold labelling of isolated plasma membranes generates point patterns that enable mapping of protein and lipid distributions. 2D spatial...